A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson

By David K. Gardner, Michelle Lane, Andrew J. Watson

This e-book pulls jointly the entire diversity of mobilephone tradition, biochemical, microscopic, and genetic strategies to check the early mammalian embryo. beforehand, there hasn't ever been this type of entire compendium, although there were extra targeted books of protocol, resembling Manipulating the Mouse Embryo, from chilly Spring Harbor. This e-book is meant to attract all constituencies, from uncomplicated experimental technological know-how to medical and animal technology purposes.

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Cantley, T. , Murphy, C. , Prather, R. , and Day, B. N. (2000). Theriogenology, 54, 787. 33. Goff, A. , and Miron, P. (2001). Reprod. Domest. , 36, 19. 34. , and Cognie, Y. (2001). Theriogenology, 54, 209. 35. Ehrmann, R. , and Gey, G. O. (1956). J. Natl. , 16, 1375. 36. , and Mauchamp, J. (1981). J. , 91, 157. 37. , and Kato, S. (1994). J. Reprod. , 100, 333. 38. , Emery, B. , and Carrell, D. T. (2001). Biol. , 64, 375. 39. , Mobberley, M. , Moore, P. , Margara, R. , and Winston, R. M. (1997).

Dry straws with a Kimwipe and cut one end of the straw with sterile scissors. Hold the cut end of the straw over an empty tube and cut the plugged end of the straw and collect the semen into the tube. Using a pipette, gently layer 200 ml of the semen under each 1 ml of sperm Talp. 5 h. During this time, transfer cumulus-enclosed oocytes from the maturation dishes to the fertilization dishes. 5°C. Gently swirl the dish to wash the oocytes. Repeat the wash. Remove fertilization dishes from the incubator and wash the cumulus-enclosed oocytes once in modified fert Talp and then transfer them to the fertilization drops.

11. Pull the uterus taut. 12. Using the 27-G needle, make a hole in the uterus around one-third of the way down from the oviduct. Ensure that the hole goes into the lumen of the uterus and not into the muscle walls. Remove the needle and put aside while keeping your eye on the hole. 13. Insert the pipette into the hole made with the needle. Ensure that the pipette is inside the lumen by gently moving the pipette up and down inside the uterus. If there is no resistance, then the pipette is in the lumen.

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