Advances In Biochemical Engineering - Biotechnology - Modern

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177. 178. 179. 180. 181. 182. 183. 184. 185. 186. 187. 188. 189. 190. 191. 192. 193. 194. 195. 196. 197. 198. 199. 200. 201. 202. 203. 204. 205. , Teslova 30, 1000 Ljubljana, Slovenia Boehringer Ingelheim Austria GmbH, Dr. Boehringer Gasse 5–11, 1121 Vienna, Austria Monolithic supports represent a novel type of stationary phases for liquid and gas chromatography, for capillary electrochromatography, and as supports for bioconversion and solid phase synthesis. As opposed to individual particles packed into chromatographic columns, monolithic supports are cast as continuous homogeneous phases.

The methacrylate- 32 F. Svec Fig. 22. Schematics for the preparation of monolithic capillary columns based polymers are stable even under extreme pH conditions such as pH 2 or 12 [144]. The sulfonic acid functionalities of the monolithic polymer remain dissociated over this pH range creating a flow velocity sufficient to achieve the desired separations in a short period of time. In contrast to the stationary phase, the analytes are uncharged, yielding symmetrical peaks. It should be noted that such extreme pH conditions especially in the alkaline range cannot be tolerated by typical silica-based packings.

51 52 53 54 55 55 Advances in Biochemical Engineering/ Biotechnology, Vol. 76 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 2002 50 A. Strancar et al. 3 Preparation of SMC and Scale-Up Strategies . . . . . . 2 Synthesis of the SMC . . . . . . . . . . . . . 62 Preparation of Large Scale SMC . . . . . . . . . . 63 4 Characteristics and Application of SMC in the Liquid Chromatography of Biomolecules . . . . . .

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