Biocatalysis - From Discovery to Application by David C. Demirjian, Pratik C. Shah (auth.), Prof. Dr.

By David C. Demirjian, Pratik C. Shah (auth.), Prof. Dr. Wolf-Dieter Fessner, A. Archelas, D. C. Demirjian, R. Furstoss, H. Griengl, K. -E. Jaeger, E. Morís-Varas, R. Öhrlein, M. T. Reetz, J.-L. Reymond, M. Schmidt, S. Servi, P. C. Shah, W. Tischer, F. Wedek

Catalytic tactics are essentially the main reasonable potential to impression selective methods in natural synthesis. For the education of enantiomerically natural compounds, the usage of enzymes is very beautiful as a result of excessive selectivities and delicate, environmentally benign response stipulations. benefiting from advances in molecular biology, targeted new enzymes at the moment are easily available in volume with homes which are amenable to amendment on call for. This quantity brings jointly best individuals from the vanguard of this interesting know-how. of their authoritative and well timed rewies they conceal the cutting-edge of biocatalysis from the invention of novel enzymes - by means of glossy screening, evolutionary or immunological methods - via immobilization recommendations for technical procedures, to their use within the uneven synthesis of vital goal compounds. hence, numerous chapters illustrate the factitious strength of lately rising biocatalysts within the phospholipid, epoxide, cyanohydrin, and oligosaccharide fields.

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The combination of this technique with those described above to introduce point mutations at a low and controlled rate [36] allows the simultaneous permutation of both single mutations and large blocks of DNA sequences. Mutants with improved properties can be identified and the genes can then be reshuffled against each other or against the wild-type gene which would correspond to the backcross-technique known from classical genetics. This process may eventually lead to an elimination of neutral and deleterious mutations from the gene pool.

During the synthesis of certain derivatives required in the synthetic sequence leading to the final drug, p-nitrobenzyl alcohol (pNB-OH) is routinely used to protect carboxylic acid functionality. Large amounts of zinc salts are employed in the final deprotection step [62]. In order Superior Biocatalysts by Directed Evolution 47 to develop an economically attractive alternative, the possibility of enzymatic deprotection based on an esterase was explored [62] (Fig. 6). Extensive searching of microorganisms led to the identification of “p-nitrobenzyl esterase” (p-NB esterase) from B.

E. kcat/Km = 256. In 85% DMF a value of kcat/Km = 131 was determined. The wildtype subtilisin E and several of the mutants were also tested as catalysts in the synthesis of poly(L-methionine) from L-methionine methyl ester in 70% DMF (Fig. 5). Whereas under these conditions the wild-type enzyme produced no detectable amount of polymer, the PC3 mutant led to significant amounts of product. Fig. 5. Polymerizing amide formation catalyzed by mutant subtilisin E Even more active subtilisin mutants were obtained upon further rounds of mutagenesis [60].

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