Ciba Foundation Symposium 84 - Microenvironments in

Content:
Chapter 1 advent: signs, Receptors and Repertoire in Haemopoietic Differentiation (pages 1–4): Melvyn F. Greaves
Chapter 2 Haemopoiesis in Mammalian Bone Marrow (pages 5–21): L. Weiss
Chapter three Self?Renewing Haemopoietic Progenitor Cells and the criteria Controlling Proliferation and Differentiation (pages 22–37): T. M. Dexter
Chapter four Haemopoietic Microenvironments in vitro: Ultras Tructural features (pages 38–67): T. D. Allen
Chapter five law and Localization of Lymphocyte creation within the Bone Marrow (pages 68–86): D. G. Osmond, M. T. E. Fahlman, G. M. Fulop and D. M. Rahal
Chapter 6 Environmental components in Haemopoietic Failure in people (pages 87–108): E. C. Gordon?Smith and M. Y. Gordon
Chapter 7 Mapping phone floor Antigen Expression of Haemopoietic Progenitor Cells utilizing Monoclonal Antibodies (pages 109–129): Melvyn Greaves, Jean Robinson, Domenico Delia, Robert Sutherland, Roland Newman and Colin Sieff
Chapter eight Lymphoid Differentiation in vitro (pages 130–160): J. W. Schrader, P. F. Bartlett, I. Clark?Lewis and A. W. Boyd
Chapter nine Microanatomy of the Thymus: Its courting to T mobile Differentiation (pages 161–177): Robert V. Rouse and Irving L. Weissman
Chapter 10 Expression and serve as of significant Histocompatibility advanced Antigens within the constructing Thymus: stories on general and Nude Mice (pages 178–192): E. J. Jenkinson
Chapter eleven The Human Thymic Microenvironment (pages 193–214): G. Janossy, J. A. Thomas, G. Goldstein and F. J. Bollum
Chapter 12 mobile and Molecular indications in T mobilephone Differentiation (pages 215–245): Jean?Francois Bach and Martine Papiernik
Chapter thirteen Histophysiology of Follicular constructions and Germinal Centres in terms of B telephone Differentiation (pages 246–264): P. Nieuwenhuis, N. A. Gastkemper and D. Opstelten
Chapter 14 The position of Germinal Centres within the iteration of Immunological reminiscence (pages 265–280): G. G. B. Klaus and Annalisa Kunkl
Chapter 15 Antigen?Presenting Cells together with Langerhans Cells, Veiled Cells and Interdigitating Cells (pages 281–301): Brigid M. Balfour, H. A. Drexhage, E. W. A. Kamperdijk and Elisabeth Ch. M. Hoefsmit
Chapter sixteen Differentiation of functionality between Antigen?Presenting Cells (pages 302–334): J. H. Humphrey
Chapter 17 Chairman's Summing?Up (pages 335–336): Melwn F. Greaves

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Extra resources for Ciba Foundation Symposium 84 - Microenvironments in Haemopoietic and Lymphoid Differentiation

Example text

However, Dr G. Johnson (personal communication) has preliminary evidence that when GM-CFC are plated in PWM-stimulated conditioned medium (as a source of GM-CSF) plus conditioned medium derived from long-term, actively haemopoietic marrow cultures, extensive self-renewal of GM-CFC can occur. Dr Johnson found that when colonies produced in this medium were re-plated, numerous secondary GM-CFC were produced. This is the first report to indicate self-renewal of GM-CFC isolated from fresh bone marrow cells.

J Cell Physiol 69: 177-184 Schruder: May I mention some characteristics of our P (persisting) cells (Schrader et a1 1980, 1981, Schrader 1981, and p 137), which resemble in many ways the self-renewing progenitor cells you have described? We derived lines of P cells from normal tissues of mice (bone marrow, thymus, lymph nodes or spleen) by culturing cell suspensions in the presence of a T cell-derived growth factor. Thus, in contrast to your experience, we obtained factor-dependent lines directly from normal tissues and not just from long-term bone marrow cultures.

In the spleen I have found macrophages and branching reticular cells. The argyrophilic reticulum in the spleen is very heavy and virtually all the branched cells are associated with it. In the bone marrow the reticular fibres BONE MARROW HAEMOPOIESIS 21 are rather light or sometimes not present, and the dark branched multinucleated cell in bone marrow is not associated with reticular fibres. With regard to cytochemistry, the dark, branched cells are peroxidasenegative and acid phosphatase-negative in their multinucleate phase.

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